MersV1, Main, Exploration, bibRecord, 001216

Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.

Identifieur interne : 001216 ( Main/Exploration ); précédent : 001215; suivant : 001217

Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.

Auteurs : Thomas Luke [États-Unis] ; Hua Wu [États-Unis] ; Jincun Zhao [République populaire de Chine] ; Rudragouda Channappanavar [États-Unis] ; Christopher M. Coleman [États-Unis] ; Jin-An Jiao [États-Unis] ; Hiroaki Matsushita [États-Unis] ; Ye Liu [États-Unis] ; Elena N. Postnikova [États-Unis] ; Britini L. Ork [États-Unis] ; Gregory Glenn [États-Unis] ; David Flyer [États-Unis] ; Gabriel Defang [Égypte] ; Kanakatte Raviprakash [Égypte] ; Tadeusz Kochel [États-Unis] ; Jonathan Wang [États-Unis] ; Wensheng Nie [États-Unis] ; Gale Smith [États-Unis] ; Lisa E. Hensley [États-Unis] ; Gene G. Olinger [États-Unis] ; Jens H. Kuhn [États-Unis] ; Michael R. Holbrook [États-Unis] ; Reed F. Johnson [États-Unis] ; Stanley Perlman [États-Unis] ; Eddie Sullivan [États-Unis] ; Matthew B. Frieman [États-Unis]

Source :

Science translational medicine [ 1946-6242 ] ; 2016.

RBID : pubmed:26888429

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Neutralizing (immunology), Antibody-Dependent Enhancement, Cattle, Chromosomes, Mammalian (genetics), Dipeptidyl Peptidase 4 (metabolism), Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin G (immunology), Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (physiology), Neutralization Tests, RNA, Messenger (genetics), RNA, Messenger (metabolism), Transduction, Genetic, Vaccination, Virus Replication.
MESH :
- chemical , genetics : RNA, Messenger.
- chemical , immunology : Antibodies, Neutralizing, Immunoglobulin G.
- genetics : Chromosomes, Mammalian, Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Dipeptidyl Peptidase 4, RNA, Messenger.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- Animals, Antibody-Dependent Enhancement, Cattle, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Inbred BALB C, Neutralization Tests, Transduction, Genetic, Vaccination, Virus Replication.

Abstract

As of 13 November 2015, 1618 laboratory-confirmed human cases of Middle East respiratory syndrome coronavirus (MERS-CoV) infection, including 579 deaths, had been reported to the World Health Organization. No specific preventive or therapeutic agent of proven value against MERS-CoV is currently available. Public Health England and the International Severe Acute Respiratory and Emerging Infection Consortium identified passive immunotherapy with neutralizing antibodies as a treatment approach that warrants priority study. Two experimental MERS-CoV vaccines were used to vaccinate two groups of transchromosomic (Tc) bovines that were genetically modified to produce large quantities of fully human polyclonal immunoglobulin G (IgG) antibodies. Vaccination with a clade A γ-irradiated whole killed virion vaccine (Jordan strain) or a clade B spike protein nanoparticle vaccine (Al-Hasa strain) resulted in Tc bovine sera with high enzyme-linked immunosorbent assay (ELISA) and neutralizing antibody titers in vitro. Two purified Tc bovine human IgG immunoglobulins (Tc hIgG), SAB-300 (produced after Jordan strain vaccination) and SAB-301 (produced after Al-Hasa strain vaccination), also had high ELISA and neutralizing antibody titers without antibody-dependent enhancement in vitro. SAB-301 was selected for in vivo and preclinical studies. Administration of single doses of SAB-301 12 hours before or 24 and 48 hours after MERS-CoV infection (Erasmus Medical Center 2012 strain) of Ad5-hDPP4 receptor-transduced mice rapidly resulted in viral lung titers near or below the limit of detection. Tc bovines, combined with the ability to quickly produce Tc hIgG and develop in vitro assays and animal model(s), potentially offer a platform to rapidly produce a therapeutic to prevent and/or treat MERS-CoV infection and/or other emerging infectious diseases.

DOI: 10.1126/scitranslmed.aaf1061
PubMed: 26888429

Affiliations:

Le document en format XML

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<author><name sortKey="Matsushita, Hiroaki" sort="Matsushita, Hiroaki" uniqKey="Matsushita H" first="Hiroaki" last="Matsushita">Hiroaki Matsushita</name>
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<author><name sortKey="Liu, Ye" sort="Liu, Ye" uniqKey="Liu Y" first="Ye" last="Liu">Ye Liu</name>
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<author><name sortKey="Postnikova, Elena N" sort="Postnikova, Elena N" uniqKey="Postnikova E" first="Elena N" last="Postnikova">Elena N. Postnikova</name>
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<author><name sortKey="Glenn, Gregory" sort="Glenn, Gregory" uniqKey="Glenn G" first="Gregory" last="Glenn">Gregory Glenn</name>
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<author><name sortKey="Flyer, David" sort="Flyer, David" uniqKey="Flyer D" first="David" last="Flyer">David Flyer</name>
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<author><name sortKey="Defang, Gabriel" sort="Defang, Gabriel" uniqKey="Defang G" first="Gabriel" last="Defang">Gabriel Defang</name>
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<country xml:lang="fr">Égypte</country>
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</affiliation>
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<author><name sortKey="Raviprakash, Kanakatte" sort="Raviprakash, Kanakatte" uniqKey="Raviprakash K" first="Kanakatte" last="Raviprakash">Kanakatte Raviprakash</name>
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<wicri:regionArea>Department of Virology, Naval Medical Research Unit-3, Cairo FPO AP 09835</wicri:regionArea>
<wicri:noRegion>Cairo FPO AP 09835</wicri:noRegion>
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<author><name sortKey="Kochel, Tadeusz" sort="Kochel, Tadeusz" uniqKey="Kochel T" first="Tadeusz" last="Kochel">Tadeusz Kochel</name>
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</placeName>
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<author><name sortKey="Wang, Jonathan" sort="Wang, Jonathan" uniqKey="Wang J" first="Jonathan" last="Wang">Jonathan Wang</name>
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<author><name sortKey="Nie, Wensheng" sort="Nie, Wensheng" uniqKey="Nie W" first="Wensheng" last="Nie">Wensheng Nie</name>
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<author><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
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<author><name sortKey="Olinger, Gene G" sort="Olinger, Gene G" uniqKey="Olinger G" first="Gene G" last="Olinger">Gene G. Olinger</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
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</placeName>
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<author><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H" last="Kuhn">Jens H. Kuhn</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
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<author><name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R" last="Holbrook">Michael R. Holbrook</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
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<author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
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<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, IA 52242</wicri:regionArea>
<placeName><region type="state">Iowa</region>
<settlement type="city">Iowa City</settlement>
</placeName>
<orgName type="university">Université de l'Iowa</orgName>
</affiliation>
</author>
<author><name sortKey="Sullivan, Eddie" sort="Sullivan, Eddie" uniqKey="Sullivan E" first="Eddie" last="Sullivan">Eddie Sullivan</name>
<affiliation wicri:level="2"><nlm:affiliation>SAB Biotherapeutics Inc., Sioux Falls, SD 57104, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>SAB Biotherapeutics Inc., Sioux Falls, SD 57104</wicri:regionArea>
<placeName><region type="state">Dakota du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B" last="Frieman">Matthew B. Frieman</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201</wicri:regionArea>
<placeName><region type="state">Maryland</region>
<settlement type="city">College Park (Maryland)</settlement>
</placeName>
<orgName type="university">Université du Maryland</orgName>
</affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.</title>
<author><name sortKey="Luke, Thomas" sort="Luke, Thomas" uniqKey="Luke T" first="Thomas" last="Luke">Thomas Luke</name>
<affiliation wicri:level="2"><nlm:affiliation>Viral and Rickettsial Diseases Department, Navy Medical Research Center, The Henry Jackson Foundation for the Advancement of Military Medicine, Silver Spring, MD 20910, USA. thomas.c.luke.ctr@mail.mil.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
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<placeName><region type="state">Maryland</region>
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<author><name sortKey="Wu, Hua" sort="Wu, Hua" uniqKey="Wu H" first="Hua" last="Wu">Hua Wu</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>SAB Biotherapeutics Inc., Sioux Falls, SD 57104</wicri:regionArea>
<placeName><region type="state">Dakota du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
<orgName type="university">Université de l'Iowa</orgName>
<placeName><settlement type="city">Iowa City</settlement>
<region type="state">Iowa</region>
</placeName>
</affiliation>
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<author><name sortKey="Channappanavar, Rudragouda" sort="Channappanavar, Rudragouda" uniqKey="Channappanavar R" first="Rudragouda" last="Channappanavar">Rudragouda Channappanavar</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, IA 52242</wicri:regionArea>
<placeName><region type="state">Iowa</region>
<settlement type="city">Iowa City</settlement>
</placeName>
<orgName type="university">Université de l'Iowa</orgName>
</affiliation>
</author>
<author><name sortKey="Coleman, Christopher M" sort="Coleman, Christopher M" uniqKey="Coleman C" first="Christopher M" last="Coleman">Christopher M. Coleman</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201</wicri:regionArea>
<placeName><region type="state">Maryland</region>
<settlement type="city">College Park (Maryland)</settlement>
</placeName>
<orgName type="university">Université du Maryland</orgName>
</affiliation>
</author>
<author><name sortKey="Jiao, Jin An" sort="Jiao, Jin An" uniqKey="Jiao J" first="Jin-An" last="Jiao">Jin-An Jiao</name>
<affiliation wicri:level="2"><nlm:affiliation>SAB Biotherapeutics Inc., Sioux Falls, SD 57104, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>SAB Biotherapeutics Inc., Sioux Falls, SD 57104</wicri:regionArea>
<placeName><region type="state">Dakota du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsushita, Hiroaki" sort="Matsushita, Hiroaki" uniqKey="Matsushita H" first="Hiroaki" last="Matsushita">Hiroaki Matsushita</name>
<affiliation wicri:level="2"><nlm:affiliation>SAB Biotherapeutics Inc., Sioux Falls, SD 57104, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>SAB Biotherapeutics Inc., Sioux Falls, SD 57104</wicri:regionArea>
<placeName><region type="state">Dakota du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Ye" sort="Liu, Ye" uniqKey="Liu Y" first="Ye" last="Liu">Ye Liu</name>
<affiliation wicri:level="2"><nlm:affiliation>Novavax Inc., Gaithersburg, MD 20878, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Novavax Inc., Gaithersburg, MD 20878</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Postnikova, Elena N" sort="Postnikova, Elena N" uniqKey="Postnikova E" first="Elena N" last="Postnikova">Elena N. Postnikova</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ork, Britini L" sort="Ork, Britini L" uniqKey="Ork B" first="Britini L" last="Ork">Britini L. Ork</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Glenn, Gregory" sort="Glenn, Gregory" uniqKey="Glenn G" first="Gregory" last="Glenn">Gregory Glenn</name>
<affiliation wicri:level="2"><nlm:affiliation>Novavax Inc., Gaithersburg, MD 20878, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Novavax Inc., Gaithersburg, MD 20878</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Flyer, David" sort="Flyer, David" uniqKey="Flyer D" first="David" last="Flyer">David Flyer</name>
<affiliation wicri:level="2"><nlm:affiliation>Novavax Inc., Gaithersburg, MD 20878, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Novavax Inc., Gaithersburg, MD 20878</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Defang, Gabriel" sort="Defang, Gabriel" uniqKey="Defang G" first="Gabriel" last="Defang">Gabriel Defang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Naval Medical Research Unit-3, Cairo FPO AP 09835, Egypt.</nlm:affiliation>
<country xml:lang="fr">Égypte</country>
<wicri:regionArea>Department of Virology, Naval Medical Research Unit-3, Cairo FPO AP 09835</wicri:regionArea>
<wicri:noRegion>Cairo FPO AP 09835</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Raviprakash, Kanakatte" sort="Raviprakash, Kanakatte" uniqKey="Raviprakash K" first="Kanakatte" last="Raviprakash">Kanakatte Raviprakash</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Naval Medical Research Unit-3, Cairo FPO AP 09835, Egypt.</nlm:affiliation>
<country xml:lang="fr">Égypte</country>
<wicri:regionArea>Department of Virology, Naval Medical Research Unit-3, Cairo FPO AP 09835</wicri:regionArea>
<wicri:noRegion>Cairo FPO AP 09835</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kochel, Tadeusz" sort="Kochel, Tadeusz" uniqKey="Kochel T" first="Tadeusz" last="Kochel">Tadeusz Kochel</name>
<affiliation wicri:level="2"><nlm:affiliation>Viral and Rickettsial Diseases Department, Navy Medical Research Center, Silver Spring, MD 20910, USA. thomas.c.luke.ctr@mail.mil.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Viral and Rickettsial Diseases Department, Navy Medical Research Center, Silver Spring, MD 20910</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Jonathan" sort="Wang, Jonathan" uniqKey="Wang J" first="Jonathan" last="Wang">Jonathan Wang</name>
<affiliation wicri:level="2"><nlm:affiliation>Thermo Fisher Scientific, South San Francisco, CA 94080, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Thermo Fisher Scientific, South San Francisco, CA 94080</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nie, Wensheng" sort="Nie, Wensheng" uniqKey="Nie W" first="Wensheng" last="Nie">Wensheng Nie</name>
<affiliation wicri:level="2"><nlm:affiliation>Thermo Fisher Scientific, South San Francisco, CA 94080, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Thermo Fisher Scientific, South San Francisco, CA 94080</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Smith, Gale" sort="Smith, Gale" uniqKey="Smith G" first="Gale" last="Smith">Gale Smith</name>
<affiliation wicri:level="2"><nlm:affiliation>Novavax Inc., Gaithersburg, MD 20878, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Novavax Inc., Gaithersburg, MD 20878</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Olinger, Gene G" sort="Olinger, Gene G" uniqKey="Olinger G" first="Gene G" last="Olinger">Gene G. Olinger</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H" last="Kuhn">Jens H. Kuhn</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R" last="Holbrook">Michael R. Holbrook</name>
<affiliation wicri:level="2"><nlm:affiliation>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD 21702</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, IA 52242</wicri:regionArea>
<placeName><region type="state">Iowa</region>
<settlement type="city">Iowa City</settlement>
</placeName>
<orgName type="university">Université de l'Iowa</orgName>
</affiliation>
</author>
<author><name sortKey="Sullivan, Eddie" sort="Sullivan, Eddie" uniqKey="Sullivan E" first="Eddie" last="Sullivan">Eddie Sullivan</name>
<affiliation wicri:level="2"><nlm:affiliation>SAB Biotherapeutics Inc., Sioux Falls, SD 57104, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>SAB Biotherapeutics Inc., Sioux Falls, SD 57104</wicri:regionArea>
<placeName><region type="state">Dakota du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B" last="Frieman">Matthew B. Frieman</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201</wicri:regionArea>
<placeName><region type="state">Maryland</region>
<settlement type="city">College Park (Maryland)</settlement>
</placeName>
<orgName type="university">Université du Maryland</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">Science translational medicine</title>
<idno type="eISSN">1946-6242</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Neutralizing (immunology)</term>
<term>Antibody-Dependent Enhancement</term>
<term>Cattle</term>
<term>Chromosomes, Mammalian (genetics)</term>
<term>Dipeptidyl Peptidase 4 (metabolism)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Immunoglobulin G (immunology)</term>
<term>Mice, Inbred BALB C</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Middle East Respiratory Syndrome Coronavirus (physiology)</term>
<term>Neutralization Tests</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Transduction, Genetic</term>
<term>Vaccination</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>Animaux</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Bovins</term>
<term>Chromosomes de mammifère (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term>
<term>Dipeptidyl peptidase 4 (métabolisme)</term>
<term>Facilitation dépendante des anticorps</term>
<term>Humains</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Réplication virale</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA</term>
<term>Tests de neutralisation</term>
<term>Transduction génétique</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Mammalian</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term>
<term>Chromosomes de mammifère</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps neutralisants</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term>
<term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term>
<term>Dipeptidyl peptidase 4</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Antibody-Dependent Enhancement</term>
<term>Cattle</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Mice, Inbred BALB C</term>
<term>Neutralization Tests</term>
<term>Transduction, Genetic</term>
<term>Vaccination</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Bovins</term>
<term>Facilitation dépendante des anticorps</term>
<term>Humains</term>
<term>Réplication virale</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA</term>
<term>Tests de neutralisation</term>
<term>Transduction génétique</term>
<term>Vaccination</term>
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</teiHeader>
<front><div type="abstract" xml:lang="en">As of 13 November 2015, 1618 laboratory-confirmed human cases of Middle East respiratory syndrome coronavirus (MERS-CoV) infection, including 579 deaths, had been reported to the World Health Organization. No specific preventive or therapeutic agent of proven value against MERS-CoV is currently available. Public Health England and the International Severe Acute Respiratory and Emerging Infection Consortium identified passive immunotherapy with neutralizing antibodies as a treatment approach that warrants priority study. Two experimental MERS-CoV vaccines were used to vaccinate two groups of transchromosomic (Tc) bovines that were genetically modified to produce large quantities of fully human polyclonal immunoglobulin G (IgG) antibodies. Vaccination with a clade A γ-irradiated whole killed virion vaccine (Jordan strain) or a clade B spike protein nanoparticle vaccine (Al-Hasa strain) resulted in Tc bovine sera with high enzyme-linked immunosorbent assay (ELISA) and neutralizing antibody titers in vitro. Two purified Tc bovine human IgG immunoglobulins (Tc hIgG), SAB-300 (produced after Jordan strain vaccination) and SAB-301 (produced after Al-Hasa strain vaccination), also had high ELISA and neutralizing antibody titers without antibody-dependent enhancement in vitro. SAB-301 was selected for in vivo and preclinical studies. Administration of single doses of SAB-301 12 hours before or 24 and 48 hours after MERS-CoV infection (Erasmus Medical Center 2012 strain) of Ad5-hDPP4 receptor-transduced mice rapidly resulted in viral lung titers near or below the limit of detection. Tc bovines, combined with the ability to quickly produce Tc hIgG and develop in vitro assays and animal model(s), potentially offer a platform to rapidly produce a therapeutic to prevent and/or treat MERS-CoV infection and/or other emerging infectious diseases.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
<li>Égypte</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Dakota du Sud</li>
<li>Guangdong</li>
<li>Iowa</li>
<li>Maryland</li>
</region>
<settlement><li>College Park (Maryland)</li>
<li>Iowa City</li>
<li>Jiangmen</li>
</settlement>
<orgName><li>Université de l'Iowa</li>
<li>Université du Maryland</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Maryland"><name sortKey="Luke, Thomas" sort="Luke, Thomas" uniqKey="Luke T" first="Thomas" last="Luke">Thomas Luke</name>
</region>
<name sortKey="Channappanavar, Rudragouda" sort="Channappanavar, Rudragouda" uniqKey="Channappanavar R" first="Rudragouda" last="Channappanavar">Rudragouda Channappanavar</name>
<name sortKey="Coleman, Christopher M" sort="Coleman, Christopher M" uniqKey="Coleman C" first="Christopher M" last="Coleman">Christopher M. Coleman</name>
<name sortKey="Flyer, David" sort="Flyer, David" uniqKey="Flyer D" first="David" last="Flyer">David Flyer</name>
<name sortKey="Frieman, Matthew B" sort="Frieman, Matthew B" uniqKey="Frieman M" first="Matthew B" last="Frieman">Matthew B. Frieman</name>
<name sortKey="Glenn, Gregory" sort="Glenn, Gregory" uniqKey="Glenn G" first="Gregory" last="Glenn">Gregory Glenn</name>
<name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E" last="Hensley">Lisa E. Hensley</name>
<name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R" last="Holbrook">Michael R. Holbrook</name>
<name sortKey="Jiao, Jin An" sort="Jiao, Jin An" uniqKey="Jiao J" first="Jin-An" last="Jiao">Jin-An Jiao</name>
<name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name>
<name sortKey="Kochel, Tadeusz" sort="Kochel, Tadeusz" uniqKey="Kochel T" first="Tadeusz" last="Kochel">Tadeusz Kochel</name>
<name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H" last="Kuhn">Jens H. Kuhn</name>
<name sortKey="Liu, Ye" sort="Liu, Ye" uniqKey="Liu Y" first="Ye" last="Liu">Ye Liu</name>
<name sortKey="Matsushita, Hiroaki" sort="Matsushita, Hiroaki" uniqKey="Matsushita H" first="Hiroaki" last="Matsushita">Hiroaki Matsushita</name>
<name sortKey="Nie, Wensheng" sort="Nie, Wensheng" uniqKey="Nie W" first="Wensheng" last="Nie">Wensheng Nie</name>
<name sortKey="Olinger, Gene G" sort="Olinger, Gene G" uniqKey="Olinger G" first="Gene G" last="Olinger">Gene G. Olinger</name>
<name sortKey="Ork, Britini L" sort="Ork, Britini L" uniqKey="Ork B" first="Britini L" last="Ork">Britini L. Ork</name>
<name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<name sortKey="Postnikova, Elena N" sort="Postnikova, Elena N" uniqKey="Postnikova E" first="Elena N" last="Postnikova">Elena N. Postnikova</name>
<name sortKey="Smith, Gale" sort="Smith, Gale" uniqKey="Smith G" first="Gale" last="Smith">Gale Smith</name>
<name sortKey="Sullivan, Eddie" sort="Sullivan, Eddie" uniqKey="Sullivan E" first="Eddie" last="Sullivan">Eddie Sullivan</name>
<name sortKey="Wang, Jonathan" sort="Wang, Jonathan" uniqKey="Wang J" first="Jonathan" last="Wang">Jonathan Wang</name>
<name sortKey="Wu, Hua" sort="Wu, Hua" uniqKey="Wu H" first="Hua" last="Wu">Hua Wu</name>
</country>
<country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name>
</region>
</country>
<country name="Égypte"><noRegion><name sortKey="Defang, Gabriel" sort="Defang, Gabriel" uniqKey="Defang G" first="Gabriel" last="Defang">Gabriel Defang</name>
</noRegion>
<name sortKey="Raviprakash, Kanakatte" sort="Raviprakash, Kanakatte" uniqKey="Raviprakash K" first="Kanakatte" last="Raviprakash">Kanakatte Raviprakash</name>
</country>
</tree>
</affiliations>
</record>

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Serveur d'exploration MERS

Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.

Human polyclonal immunoglobulin G from transchromosomic bovines inhibits MERS-CoV in vivo.

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